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You have a starting concentration of cells that is 6.0 x 10^8 cells/mL.

You have a starting concentration of cells that is 6.0 x 10^8 cells/mL. Calculate how to

two-fold dilution series, in which there are 5 different concentrations of cells, with the most

concentrated being 4 x 10^4/mL. You need at least 350 μL of each concentration. This is a multistep

problem. Clearly show each step. For the serial dilution, indicate (i) how much media goes

in each tube; (ii) how much is transferred from tube to tube; (iii) what the concentrations are of

each of the 5 tubes; (iv) what the DF and TDF are for each dilution tube.

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